Molecular Testing & Oncology

Molecular Testing Services

Tulsa Medical Laboratory offers a state of the art in house molecular department that focuses on women’s health testing. The Hologic Thin Prep Pap Test has proven to be the perfect media for performing molecular testing which enables the clinicians to obtain six results with one sample collection; cytology, chlamydia, gonorrhea, trichomonas vaginalis, high risk HPV and genotyping for HPV 16 and 18/45. A single one milliliter aliquot can be tested up to six times.

Molecular Testing Staff

Currently Tulsa Medical Lab offers testing for Chlamydia trachomatis, Neisseria Gonorrhea, Trichomonas vaginalis, High Risk HPV, HPV Genotyping of HPV 16 and 18/45 directly from the Thin Prep Pap test vial. In addition HSV 1 & 2 is available from the Aptima Multi test swab. Trichomonas and Chlamydia and Gonorrhea can also be tested for from the Aptima Multi test swab.

Complete automation of molecular testing is achieved by utilizing the Hologic Tomcat for transfer of the barcoded specimen from the thin prep vial to the Aptima Specimen Transport Tube. The Aptima Specimen Transport tubes are then placed on the Hologic Panther. The Hologic Panther is a random access, fully automated system that performs all the Aptima Assays that Tulsa Medical Laboratory offers. The Panther was recently upgraded to include a fluorometer which enables it to perform real time assays in addition to end point assays. End point assays take 3.5 hours to yield the first result; real time assays yield result in 2.7 hours.

Both types of assays employ the proven Aptima technology of target capture and target amplification by transcription Mediated Amplification. The assays differ in the method of detection; the end point assays utilize HPA (Hybridization Protection Assay) and a luminometer for determining the RLU’s (relative light units) of each sample. The real time assays utilize a fluorometer and fluorescent labeled probes referred to as “torches” in detecting the amplicon along with an internal control to monitor targeted nucleic acid capture, amplification and detection.

For additional information on our current testing options please contact the molecular department at (918) 281-6865.

Oncology Testing

Tulsa Medical Laboratory realizes the need for comprehensive tumor profiles and targeted biomarker tests that lead to the best possible treatment modality for each individual patient. We work with a CAP and CLIA certified national laboratory to expediently provide this information.

KRAS, BRAF, EGFR, NGS and PD-L1 testing are performed by the Saint Francis Hospital Laboratory. A wide variety of immunohistochemistry, fluorescence in situ hybridization (FISH) tests, and flow cytometry are also available. Our experienced, knowledgeable pathology staff is available to answer questions and share their vast wealth of experience with you.

For more information, please call us at (918) 281-6854.

New Molecular Testing Services

Tulsa Medical Laboratories is pleased to announce the addition of three new tests to our molecular testing menu; the Aptima Bacterial Vaginosis Assay, the Aptima Mycoplasma Genitalium Assay and the Aptima CV/TV (Candida Vaginitis/Trichomonas Vaginalis).

  • The Aptima BV assay is an FDA approved in vitro qualitative NAAT that utilizes real time TMA for detection and quantitation of ribosomal RNA from bacteria associated with bacterial vaginosis, including Lactobacillus (L.gasseri, L.crispatus, and L. jensenii), Gardnerella vaginalis and Atopobium vaginae.

The assay gives a qualitative result for BV and does not report for individual organisms.

  • The Aptima Mycoplasma genitalium assay is an FDA approved in vitro qualitative NAAT for the detection of rRNA from Mycoplasma genitalium. The assay is intented as an aid in the diagnosis of M. genitalium urogenital infections in male and female patients suspected of M. genitalium infection.
  • The CV/TV assay is an FDA approved, qualitative in vitro NAAT for the detection of RNA from microorganisms associated with vulvovaginal candidiasis and trichomoniasis. The assay uses real time TMA to detect and report results for the following organisms;
  1. Candida species group (C. albicans, C. tropicalis, C. parapsilosis, C. dublinensis)
  2. Candida glabrata
  3. Trichomonas vaginalis

These assays are not available from the thin prep pap test vial and must be collected in the Aptima Multi test swab (orange Aptima tube).  These assays will replace the current BD affirm vaginitis panel which will not be available after March 13, 2020.  The assays may be ordered individually or as a “full vaginal panel” which would be CT/NG, BV, CV/TV and M. genitalium.  We are excited to be able to offer a one-swab solution for vaginitis testing.

If you are interested in ordering supplies for these tests or have questions about the tests please feel free to contact the molecular department supervisor, Julie Gregg at (918) 281-6865.